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Direct and indirect consequences on gene expression of a thyroid hormone receptor alpha 1 mutation restricted to Sertoli cells
Author(s) -
Chatonnet Fabrice,
Livera Gabriel,
Fumel Betty,
FouchÉCourt Sophie,
Flamant Frédéric
Publication year - 2014
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22437
Subject(s) - sertoli cell , biology , thyroid hormone receptor alpha , thyroid hormone receptor , thyroid , hormone , endocrinology , medicine , fgf9 , gene , mutation , thyroid hormone receptor beta , microbiology and biotechnology , hormone receptor , nuclear receptor , spermatogenesis , genetics , transcription factor , cancer , breast cancer
SUMMARY Thyroid hormone is required for the timely transition of Sertoli cells from proliferative to differentiating and maturing. This transition takes place during a critical developmental period in mammals, which in mice is the first post‐natal week. In order to identify the underlying molecular mechanisms of this differentiation process, we used Cre/loxP technology to selectively block the function of the thyroid hormone receptor TRα1 in Sertoli cells. We then used RNA‐seq to analyze the changes in gene expression induced in the post‐natal testis. This differential analysis provides genetic clues to the initial testicular defects resulting from disrupted thyroid hormone signaling, and suggests that Sertoli cells influence germ cells soon after their birth. Mol. Reprod. Dev. 81: 1159–1166, 2014. © 2014 Wiley Periodicals, Inc .