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Identification and characterization of genes differentially expressed in X and Y sperm using suppression subtractive hybridization and cDNA microarray
Author(s) -
Chen Xiaoli,
Yue Yang,
He Yanan,
Zhu Huabin,
Hao Haisheng,
Zhao Xueming,
Qin Tong,
Wang Dong
Publication year - 2014
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22386
Subject(s) - biology , suppression subtractive hybridization , sperm , genetics , gene , contig , microarray analysis techniques , microarray , gene expression profiling , complementary dna , dna microarray , expressed sequence tag , gene expression , cdna library , genome
SUMMARY Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH), cDNA microarray, and sequence‐homology analysis. Microarray data and Significance Analysis of Microarrays software were used to identify 31 differentially expressed genes, only four of which were previously identified. These genes are involved in fundamental life processes of mature sperm, and may be associated with the differences between X and Y sperm since 27 versus 4 were upregulated in X versus Y sperm, respectively. The levels of expression of seven genes—including the known genes UTY , DPH3 , CYTB , and ISCU , and the unknown genes X + Y contig 41, X + Y contig 18 , and Y + X contig 16 —were validated by quantitative real‐time PCR, and some genes were clearly differentially expressed by X and Y sperm, despite the presence of intercellular bridges among spermatids. These results provide a theoretical basis for research on gene expression during sperm development, as well as on sex control at the level of sperm. Mol. Reprod. Dev. 81: 908–917, 2014. © 2014 Wiley Periodicals, Inc .