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Molecular Reproduction & Development Volume 79, Issue 6, June 2012 cover image
Publication year - 2012
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22369
Subject(s) - biology , oocyte , luteinizing hormone , follicle , hormone , cyclic adenosine monophosphate , adenosine , follicle stimulating hormone , endocrinology , medicine , reproduction , ovulation , microbiology and biotechnology , andrology , biochemistry , genetics , receptor , embryo
Abstract Maturation in bovine oocytes is triggered by a single surge of gonadotropins, i.e., luteinizing hormone (LH) and follicle‐stimulating hormone (FSH), which cause changes in the intraooplasmic concentrations of second messengers such as cyclic adenosine 3′,5′‐ monophosphate and Ca 2 + through their activity on cumulus cells (see Silvestre et al., this issue). This time‐sequence shows a representative fluorescent intensity profile of the Ca 2 + wave triggered in cumulus cells by the addition of FSH in an immature bovine cumulusoocyte complex loaded with the fluorescent Ca 2 + indicator Fluo‐4 FF/AM. The top image shows the cumulus‐oocyte complex at the time of FSH addition, and the bottom image shows the same cumulus‐oocyte complex 162 sec after exposure; intermediate images were taken at 48, 66, 84, 108, 132 sec from the addition of FSH, respectively.