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Characterization and in vitro differentiation potency of early‐passage canine amnion‐ and umbilical cord‐derived mesenchymal stem cells as related to gestational age
Author(s) -
Filioli Uranio Manuel,
Dell'Aquila Maria Elena,
Caira Michele,
Guaricci Antonio Ciro,
Ventura Mario,
Catacchio Claudia RITA,
Martino Nicola Antonio,
Valentini Luisa
Publication year - 2014
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22322
Subject(s) - biology , mesenchymal stem cell , stem cell , immunology , amniotic stem cells , andrology , umbilical cord , cd44 , embryonic stem cell , population , microbiology and biotechnology , adult stem cell , cell , genetics , medicine , gene , environmental health
SUMMARY Fetal adnexa are a non‐controversial source of mesenchymal stem cells (MSCs) that have high plasticity, a high proliferation rate, and the ability to differentiate towards multiple lineages. MSC populations have been characterized for their stemness and differentiation capabilities; more recent work has focused on MSC selection and on establishing predictable elements to discriminate the cells with the most potential for regenerative medicine. In this study, we cytogenetically and molecularly characterized and followed the in vitro proliferation and differentiation potential of early‐passage canine amniotic membrane MSCs (AM‐MSCs) and umbilical cord matrix MSCs (UCM‐MSCs) isolated from fetuses at early (35–40 days) and late (45–55 days) gestational ages. We found that cells from both fetal gestational ages showed similar features. In all examined cell lines, the morphology of proliferating cells typically appeared fibroblast‐like. Population doublings, passaged up to 10 times, increased significantly with passage number. In both cell types, cell viability and chromosomal number and structure were not affected by gestational age at early passages. Passage‐3 AM‐ and UCM‐MSCs from both gestational phases also expressed embryonic ( POU5F1 ) and mesenchymal ( CD29 , CD44 ) stemness markers, whereas hematopoietic and histocompatibility markers were never found in any sample. Passage‐3 cell populations of each cell type were also multipotential as they could differentiate into neurocytes and osteocytes, based on cell morphology, specific stains, and molecular analysis. These results indicated that MSCs retrieved from the UCM and AM in the early and late fetal phases of gestation could be used for canine regenerative medicine. Mol. Reprod. Dev. 81: 539–551, 2014. © 2014 Wiley Periodicals, Inc .