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Oocyte‐secreted factors in oocyte maturation media enhance subsequent development of bovine cloned embryos
Author(s) -
Su Jianmin,
Wang Yongsheng,
Zhang Lei,
Wang Bo,
Liu Jun,
Luo Yan,
Guo Zekun,
Quan Fusheng,
Zhang Yong
Publication year - 2014
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22302
Subject(s) - biology , oocyte , embryo , in vitro maturation , blastocyst , somatic cell nuclear transfer , andrology , embryogenesis , human fertilization , embryo culture , microbiology and biotechnology , genetics , medicine
SUMMARY Successful in vitro maturation (IVM) and oocyte quality both affect the subsequent development of cloned embryos derived from somatic‐cell nuclear transfer (SCNT). Developmental competence is usually lower in oocytes matured in vitro compared with those that matured in vivo, possibly due to insufficient levels of oocyte‐secreted factors (OSFs) and disrupted oocyte–cumulus communication. This study investigated the effects of OSFs secreted by denuded oocytes (DOs) during IVM on the subsequent developmental competence of cloned bovine embryos. Cumulus–oocyte complexes (COCs) from antral follicles of slaughtered‐cow ovaries collected from an abattoir were divided into four groups: COCs co‐cultured with and without DOs in maturation media used for SCNT, as well as COCs co‐cultured with and without DOs in maturation media used for in vitro fertilization (IVF). Based on the developmental competence and embryo quality of bovine embryos generated from these four groups, we found that co‐culturing the COCs with DOs enhanced the in vitro development of IVF and cloned bovine embryos, and potentially generated more high‐quality cloned blastocysts that possessed locus‐specific histone modifications at levels similar to in vitro‐fertilized embryos. These results strongly suggest that co‐culturing COCs with DOs enhances subsequent developmental competence of cloned bovine embryo. Mol. Reprod. Dev . 81: 341–349, 2014. © 2014 Wiley Periodicals, Inc.