Premium
Molecular Reproduction & Development Volume 81, Issue 6, June 2014 Cover Image
Publication year - 2014
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22243
Subject(s) - biology , euchromatin , dna damage , confocal , dna , dna repair , confocal microscopy , microbiology and biotechnology , genetics , chromatin , evolutionary biology , heterochromatin , optics , physics
DNA double‐strand breaks are detrimental to a cell's ability to survive, but gametes have evolved mechanisms that reduce their accumulation. Singh and Raman analyzed such breaks by confocal microscopy to determine the spatio‐temporal dynamics of radiation‐induced foci in mouse round spermatids. The ‘γH2AX foci' (green) marking DNA damage in nuclear chromocenters (deep blue) tend to expand and protrude into euchromatic regions, but the DNA recovers H3K9 trimethylation (red) following damage repair.