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Decreased Expression of CD9 in Bovine Oocytes After Cryopreservation and the Relationship to Fertilization Capacity
Author(s) -
Zhou GuangBin,
Zeng Yan,
Meng QingGang,
Liu Ying,
Dai YunPing,
Zhu ShiEn,
Bunch Thomas D.,
Hou YunPeng
Publication year - 2013
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22181
Subject(s) - human fertilization , biology , andrology , zona pellucida , vitrification , cryopreservation , oocyte , sperm , in vitro fertilisation , western blot , embryo , microbiology and biotechnology , anatomy , gene , genetics , medicine
SUMMARY This study was conducted to investigate the effect of vitrification of bovine metaphase‐II (MII) oocytes on CD9 expression and fertilization capacity. Surviving vitrified/warmed oocytes were used to detect CD9 distribution (fluorescence microscopy), CD9 mRNA (qRT‐PCR), and CD9 protein expression (Western blot), and to analyze in vitro fertilization rates (number of sperm bound to or that penetrated the oocytes) after removing the zona pellucida. Fresh oocytes acted as control. The experimental results showed that the vitrification/warming procedures significantly decreased CD9 expression at the mRNA and protein levels, and changed the CD9 distribution pattern in bovine oocytes. After fertilization in vitro, the average number of sperm binding and penetration of vitrified oocytes were significantly lower than those of the non‐vitrified oocytes. In conclusion, vitrification of bovine oocytes caused a decrease in CD9 expression at the mRNA and protein levels, and an alteration of CD9 distribution pattern, which may have resulted in lowered fertilization capacity. Mol. Reprod. Dev. 80: 451–459, 2013. © 2013 Wiley Periodicals, Inc .