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The ability to achieve meiotic maturation in the dog oocyte is linked to glycolysis and glutamine oxidation
Author(s) -
Songsasen Nucharin,
Wesselowski Sonya,
Carpenter James W.,
Wildt David E.
Publication year - 2012
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22011
Subject(s) - oocyte , germinal vesicle , biology , glutamine , glycolysis , follicle , meiosis , in vitro maturation , andrology , metabolism , ovarian follicle , medicine , endocrinology , ovary , embryo , microbiology and biotechnology , biochemistry , gene , amino acid
We tested the hypothesis that meiotic competence of dog oocytes is tightly linked with donor follicle size and energy metabolism. Oocytes were recovered from small (<1 mm diameter, n = 327), medium (1–<2 mm, n = 292) or large (≥2 mm, n = 102) follicles, cultured for 0, 24, or 48 hr, and then assessed for glycolysis, glucose oxidation, pyruvate uptake, glutamine oxidation, and nuclear status. More oocytes ( P  < 0.05) from large follicles (37%) reached the metaphase‐II (MII) stage than from the small group (11%), with the medium‐sized class being intermediate (18%; P  > 0.05). Glycolytic rate increased ( P  < 0.05) as oocytes progressed from the germinal vesicle (GV) to MII stage. After 48 hr of culture, oocytes completing nuclear maturation had higher ( P  < 0.05) glycolytic rates than those arrested at earlier stages. GV oocytes recovered from large follicle oocytes had higher ( P  < 0.05) metabolism than those from smaller counterparts at culture onset. MII oocytes from large follicles oxidized more ( P  < 0.05) glutamine than the same stage gametes recovered from smaller counterparts. In summary, larger‐sized dog follicles contain a more metabolically active oocyte with a greater chance of achieving nuclear maturation in vitro. These findings demonstrate a significant role for energy metabolism in promoting dog oocyte maturation, information that will be useful for improving culture systems for rescuing intraovarian genetic material. Mol. Reprod. Dev. 79: 186–196, 2012. Published 2011. This article is a U.S. Government work and is in the public domain in the USA.

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