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Directional differentiation of chicken primordial germ cells into adipocytes, neuron‐like cells, and osteoblasts
Author(s) -
Li B.C.,
Tian Z.Q.,
Sun M.,
Xu Q.,
Wang X.Y.,
Qin Y.R.,
Xu F.,
Gao B.,
Wang K.H.,
Sun H.C.,
Chen G.H.
Publication year - 2010
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.21224
Subject(s) - biology , ibmx , alkaline phosphatase , cellular differentiation , staining , microbiology and biotechnology , oil red o , retinoic acid , adipogenesis , cell culture , in vitro , biochemistry , mesenchymal stem cell , genetics , forskolin , gene , enzyme
Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in induction media containing dexamethasone, insulin and/or 3‐isobutyl‐1‐methylxanthine (IBMX), and differentiation rates ranging from 74% to 91% were identified by oil red‐O and alkaline phosphatase (ALP) staining. For differentiation into neuron‐like cells, chicken PGCs were cultured for 3 or 7 days in the induction media containing retinoic acid (RA) and IBMX, and differentiation rates ranging from 71% to 87% were identified by toluidine blue staining and immunohistochemical staining. For differentiation into osteoblasts, chicken PGCs were cultured for 15 or 21 days in the induction media containing desamethasone, β‐glycerol phosphate and/or vitamin C, and differentiation rates ranging from 47% to 79% were confirmed by Von Kossa, cytochemical and immunohistochemical staining. These data suggest that, like mammalian PGCs, chicken PGCs can differentiate into different cell types in vitro. Mol. Reprod. Dev. 77: 795–801, 2010. © 2010 Wiley‐Liss, Inc.