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Expression of zinc finger protein 105 in the testis and its role in male fertility
Author(s) -
Zhou Huaxin,
Liu LanHsin,
Zhang Heng,
Lei Zhenmin,
Lan ZiJian
Publication year - 2010
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.21171
Subject(s) - biology , zinc finger , male fertility , fertility , microbiology and biotechnology , andrology , genetics , evolutionary biology , gene , transcription factor , population , demography , sociology , medicine
Abstract Using an in silico approach, we identified a putative zinc finger domain‐containing transcription factor (zinc finger protein 105, ZFP105) enriched in the adult mouse testis. RT‐PCR analyses showed that Zfp105 was indeed highly expressed in adult mouse testis and that its expression was regulated during postnatal development. To further characterize Zfp105 expression, we generated a Zfp105:β‐galactosidase (LacZ) knock‐in reporter mouse line ( Zfp105 LacZ/+ ) in which a Zfp105:LacZ fusion gene was expressed. Whole‐mount LacZ analyses of adult Zfp105 LacZ/+ tissues showed robust LacZ staining in the testis, very weak staining in the ovary, and no staining in the spleen, liver, kidney, heart, lung, thymus, adrenal gland, uterus, or oviduct. Sectional LacZ staining showed that ZFP105 was highly expressed in pachytene spermatocytes. ZNF35 , the human ortholog of Zfp105 , was also highly expressed in human testis. Immunofluorescence analysis showed that ZNF35 was located primarily in the cytoplasm of male germ cells. More importantly, reduced male fertility was observed in adult Zfp105 LacZ/LacZ mice. Histological studies showed the presence of undifferentiated spermatogenic cells in the lumen of seminiferous tubules at stage VII and in the epididymal lumen of adult Zfp105 LacZ/LacZ mice. Taken together, our results suggest that ZFP105 is a male germ‐cell factor and plays a role in male reproduction. Mol. Reprod. Dev. 77: 511–520, 2010. © 2010 Wiley‐Liss, Inc.