Premium
Production of quail ( Coturnix japonica ) germline chimeras derived from in vitro‐cultured gonadal primordial germ cells
Author(s) -
Park Tae Sub,
Kim Mi A,
Lim Jeong Mook,
Han Jae Yong
Publication year - 2008
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20821
Subject(s) - biology , quail , germline , chimera (genetics) , embryo , andrology , coturnix coturnix , coturnix japonica , coturnix , endocrinology , medicine , genetics , gene
A previous report from our laboratory documented successful production of quail ( Coturnix japonica ) germline chimeras by transfer of gonadal primordial germ cells (gPGCs). Subsequently, this study was designed to evaluate whether gPGCs can be maintained in vitro for extended period, and furthermore, these cultured PGCs can induce germline transmission after transfer into recipient embryos. In experiment 1, gonadal cells from the two strains (wild‐type plumage (WP) and black (D) quail) were cultured in vitro for 10 days. Using antibody QCR1, we detected a continuous, significant ( P = 0.0002) increase in the number of WP, but not D, PGCs. QCR1‐positive WP colonies began to form after 7 days in culture. On Day 10 of culture, 803 WP PGCs were present as a result of a continuous increase, whereas no D PGC colonies could be detected and the D gonadal stroma cells were rolled up. Differences in the PGCs or the gonadal stroma cells of the two different strains might account for these differences. In experiment 2, WP PGC colonies were maintained in vitro up to Day 20 of culture, and 10‐ or 20‐day‐cultured PGCs were microinjected into dorsal aortas of 181 recipient D embryos. Thirty‐five (19.3%) of the transplanted embryos hatched after incubation, and 25 (71.4%) of the hatchlings reached sexual maturity. Testcrossing of the sexually mature hatchlings resulted in three (10 days, 33.3%) and eight (20 days, 50.0%) germline chimeras respectively. This report is the first to describe successful production of germline chimera by transfer of in vitro‐cultured gPGCs in quail. Mol. Reprod. Dev. 75: 274–281, 2008. © 2007 Wiley‐Liss, Inc.