Premium
Regulation of cAMP on the first mitotic cell cycle of mouse embryos
Author(s) -
Yu Aiming,
Zhang Zhe,
Bi Qiang,
Sun Bingqi,
Su Wenhui,
Guan Yifu,
Mu Runqing,
Miao Changsheng,
Zhang Jie,
Yu Bingzhi
Publication year - 2008
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20782
Subject(s) - maturation promoting factor , protein kinase a , biology , anaphase , microbiology and biotechnology , metaphase , mitosis , mitogen activated protein kinase kinase , wee1 , kinase , cell cycle , cyclin dependent kinase 1 , biochemistry , cell , gene , chromosome
Mitosis promoting factor (MPF) plays a central role during the first mitosis of mouse embryo. We demonstrated that MPF activity increased when one‐cell stage mouse embryo initiated G2/M transition following the decrease of cyclic adenosine 3′, 5′‐monophosphate (cAMP) and cAMP‐dependent protein kinase (PKA) activity. When cAMP and PKA activity increases again, MPF activity decreases and mouse embryo starts metaphase–anaphase transition. In the downstream of cAMP/PKA, there are some effectors such as polo‐like kinase 1 (Plk1), Cdc25, Mos (mitogen‐activated protein kinase kinase kinase), MEK (mitogen‐activated protein kinase kinase), mitogen‐activated protein kinase (MAPK), Wee1, anaphase‐promoting complex (APC), and phosphoprotein phosphatase that are involved in the regulation of MPF activity. Here, we demonstrated that following activation of MPF, MAPK activity was steady, whereas Plk1 activity fluctuated during the first cell cycle. Plk1 activity was the highest at metaphase and decreased at metaphase–anaphase transition. Further, we established a mathematical model using Gepasi algorithm and the simulation was in agreement with the experimental data. Above all the evidences, we suggested that cAMP and PKA might be the upstream factors which were included in the regulation of the first cell cycle development of mouse embryo. Mol. Reprod. Dev. 75: 489–495, 2008. © 2007 Wiley‐Liss, Inc.