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The PKC pathway and in particular its β1 isoform is clearly involved in meiotic arrest maintenance but poorly in FSH‐induced meiosis resumption of the mouse cumulus cell enclosed oocyte
Author(s) -
Denys Anne,
Avazeri Nathalie,
Lefèvre Brigitte
Publication year - 2007
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20748
Subject(s) - oocyte , meiosis , biology , oocyte activation , protein kinase c , microbiology and biotechnology , chromosomal translocation , calphostin c , cytoplasm , andrology , signal transduction , endocrinology , genetics , gene , medicine , embryo
PKC modulators were used to investigate the role of the PKC pathway either on the maintenance of meiotic arrest or on FSH‐induced maturation of mouse cumulus cell enclosed oocytes (CEOs). (1) Whereas PKC activation (PMA 8 µM) overcomed clearly the HX‐maintained meiotic arrest (83.7 ± 3.6% vs. 16.1 ± 10.6% GVBD oocytes), PKC inhibition (Calphostin C 100 nM) did not. On the contrary, it better maintained the meiotic arrest than HX alone. (2) No significant effect of PKC activation or inhibition was observed. (3) HX alone maintained PKCβ1 in the cytoplasm, whereas FSH and PKC activation induced partly its translocation into the nucleus. The results show that whereas the PKC pathway is clearly involved in maintenance of the meiotic arrest through PKCβ1, it is not involved in FSH‐induced meiosis of CEOs. Mol. Reprod. Dev. 74: 1575–1580, 2007. © 2007 Wiley‐Liss, Inc.