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Distribution and expression of phosphorylated histone H3 during porcine oocyte maturation
Author(s) -
Gu Ling,
Wang Qiang,
Wang ChunMing,
Hong Yan,
Sun ShaoGuang,
Yang ShuYan,
Wang JiGui,
Hou Yi,
Sun QingYuan,
Liu WeiQuan
Publication year - 2008
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20706
Subject(s) - germinal vesicle , biology , histone h3 , oocyte , histone , microbiology and biotechnology , phosphorylation , chromatin , meiosis , dephosphorylation , metaphase , genetics , chromosome , embryo , phosphatase , dna , gene
Phosphorylation modification of core histones is correlated well with diverse chromatin‐based cell activities. However, its distribution pattern and primary roles during mammalian oocyte meiosis are still in dispute. In this study, by performing immunofluorescence and Western blotting, spatial distribution and temporal expression of phosphorylated serine 10 or 28 on histone H3 during porcine oocyte meiotic maturation were examined and distinct subcellular distribution patterns between them were presented. Low expression of phosphorylated H3/ser10 was detected in germinal vesicle. Importantly, following gradual dephosphorylation from germinal vesicle (GV) to late germinal vesicle (L‐GV) stage, a transient phosphorylation at the periphery of condensed chromatin was re‐established at early germinal vesicle breakdown (E‐GVBD) stage, and then the dramatically increased signals covered whole chromosomes from pre‐metaphase I (Pre‐MI) to metaphase II (MII). Similarly, hypophosphorylation of serine 28 on histone H3 was also monitored from GV to E‐GVBD, indicating dephosphorylation of histone H3 maybe involved in the regulation of meiotic resumption. Moreover, the rim staining on the chromosomes and high levels of H3/ser28 phosphorylation were observed in Pre‐MI, MI, and MII stage oocytes. Based on above results, such stage‐dependent dynamics of phosphorylation of H3/ser 10 and 28 may play specific roles during mammalian oocyte maturation. Mol. Reprod. Dev. 75: 143–149, 2008. © 2007 Wiley‐Liss, Inc.