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Angiotensin I‐converting enzyme insertion‐related genotypes and allele are associated with higher susceptibility of endometriosis and leiomyoma
Author(s) -
Hsieh YaoYuan,
Lee ChengChun,
Chang ChiChen,
Wang YuKuo,
Yeh LianShun,
Lin ChichSheng
Publication year - 2007
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20474
Subject(s) - genotype , biology , endometriosis , allele , leiomyoma , uterine leiomyoma , angiotensin converting enzyme , allele frequency , restriction fragment length polymorphism , medicine , endocrinology , gene , genetics , pathology , uterus , blood pressure
Endometriosis and leiomyoma display features similar to malignancy, requiring neovascularization to proliferation and growth. Altered vascular‐related genes might be related to the development of endometriosis and leiomyoma. Polymorphisms of the angiotensin‐converting enzyme (ACE) insertion/deletion (I/D) genes have been linked with some vascular diseases. This study investigates whether ACE I/D gene polymorphisms could be used as markers of susceptibility in endometriosis and leiomyoma. Women were divided into three groups: (1) endometriosis (n = 125); (2) leiomyoma (n = 120); (3) normal controls (n = 128). Genomic DNA was obtained from peripheral leukocyte. ACE I/D gene polymorphisms in intron 16 were amplified by polymerase chain reaction and restriction fragment length polymorphism (PCR‐RFLP) Genotypes and allelic frequencies in both groups were compared. We observed the genotype distribution and allele frequency of ACE I/D gene polymorphisms in both groups were significantly different. Proportions of ACE*I homozygote/heterozygote/D homozygote in both groups were: (1) 50.4/24/25.6%; (2) 25/23.33/51.67%; (3) 10.2/29.7/60.1%. Proportions of I/D alleles in each group were: (1) 62.4/37.6%; (2) 36.7/63.3%; (3) 25/75%. We concluded that ACE*I/D gene polymorphisms are associated with endometriosis and leiomyoma susceptibilities. ACE*I‐related genotypes and allele are strongly related to the occurrence of endometriosis and moderately related to the occurrence of leiomyoma. Mol. Reprod. Dev. 74: 808–814, 2007. © 2006 Wiley‐Liss, Inc.