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In vivo effect of growth hormone on the expression of connexin‐43 in bovine ovarian follicles
Author(s) -
Kaiser Germán G.,
Kölle Sabine,
Boie Gudrun,
Sinowatz Fred,
Palma Gustavo A.,
Alberio Ricardo H.
Publication year - 2006
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20438
Subject(s) - folliculogenesis , biology , endocrinology , follicle , in vivo , medicine , growth differentiation factor 9 , andrology , connexin , ovarian follicle , tunel assay , oocyte , estrous cycle , gap junction , immunohistochemistry , hormone , microbiology and biotechnology , immunology , embryo , embryogenesis , intracellular
Abstract This study assessed the in vivo effects of recombinant growth hormone (rGH) administration on the expression of connexin‐43 (Cx43) in bovine ovarian follicles. Two independent experiments were carried out using either estrous unsynchronized or synchronized multiparous Aberdeen Angus cows. rGH‐treated animals were inoculated with a single dose of hormone (500 mg, intramuscular) while control animals were inoculated with hormone diluent. Five and 14 days after treatment (Experiments 1 and 2, respectively), ovarian Cx43 and apoptosis expression were assessed using immunohistochemistry. In both experiments primary, secondary, and tertiary follicles from rGH‐treated and control groups distinctly expressed Cx43 protein. Primordial and atretic follicles were Cx43‐negative. Interestingly, the number of Cx43 dots per granulosa cell did not show significant variation at different folliculogenesis stages neither in the rGH‐treated nor in the control group. In unsynchronized animals, Cx43‐positive follicles per total number of follicles ratio showed an interaction between stage of folliculogenesis and treatment due to significant differences between treatment groups in the early secondary follicle stage. In synchronized animals, there were significant differences between treatment groups and folliculogenesis stage. In both experiments, atretic follicles showed apoptosis‐related DNA‐fragmentation as determined by terminal uridin nick end labeling (TUNEL) assay. Tertiary follicles presented moderate TUNEL staining. Our results show significant increment in the number of ovarian follicles expressing the gap junction subunit Cx43 after in vivo rGH treatment. Therefore, we conclude that growth hormone can modulate in vivo gap junction assembly at early stages of folliculogenesis. Mol. Reprod. Dev. © 2006 Wiley‐Liss, Inc.