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Chimeric honeybees ( Apis mellifera ) produced by transplantation of embryonic cells into pre‐gastrula stage embryos and detection of chimerism by use of microsatellite markers
Author(s) -
Bergem M.,
Norberg K.,
Roseth A.,
Meuwissen T.,
Lien S.,
Aamodt R. H.
Publication year - 2006
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20435
Subject(s) - biology , chimera (genetics) , gastrulation , embryo , embryonic stem cell , transplantation , embryogenesis , andrology , microbiology and biotechnology , inner cell mass , immunology , genetics , blastocyst , gene , medicine
The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produce chimeric honeybees by use of cell transplantation. Embryonic cells were transplanted between pre‐gastrula stage embryos (32–34 hr after oviposition) and hatched larvae were reared in vitro for 4 days. Chimeric individuals were detected by use of microsatellite analysis and a conservative estimation approach. 4.8% of embryos, posteriorly injected with embryonic cells, developed into chimeric honeybee larvae. By injection of cells pre‐stained with fluorescent cell tracer dye, we studied the integration of transplanted cells in the developing embryos. Number of injected cells varied from 0 to 50 and cells remained and multiplied mainly in the area of injection. Mol. Reprod. Dev. © 2006 Wiley‐Liss, Inc.