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Bovine ICM derived cells express the Oct4 ortholog
Author(s) -
Yadav Prem S.,
Kues Wilfried A.,
Herrmann Doris,
Carnwath Joseph W.,
Niemann Heiner
Publication year - 2005
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20343
Subject(s) - biology , inner cell mass , embryonic stem cell , bovine genome , cell culture , stem cell , pou domain , microbiology and biotechnology , pseudogene , induced pluripotent stem cell , clone (java method) , sox2 , transcription factor , genetics , embryo , gene , embryogenesis , genome , blastocyst , homeobox
The goal of this study was to define conditions for the successful isolation of embryonic stem cells from bovine blastocysts. Expression of the Pit‐Oct‐Unc (POU) transcription factor Oct4 was employed to monitor the pluripotent status of cultured cells. No expression of the previously identified bovine Oct4 pseudogene was found, and transcription of the Oct4 ortholog correlated with the proliferative potential of bovine ICM derived cells. Two methods to isolate pluripotent inner cell mass were compared; 90% of trypsin isolated ICMs formed growing cultures, whereas only 12%–23% of the ICMs isolated by immunosurgery attached and grew. Colony formation from complete blastocysts was 55%. The bovine ICM derived cells could be grown for 4–7 passages. However, Oct4 transcripts were only present in the primary cultures, indicating that the initial culture period of bovine ICM derived cells is critical and needs to be optimized to yield true ES cells. In contrast to bovine ICMs, murine ICMs yielded rapidly growing cells, which proliferated for more than 60 passages. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.