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Active demethylation of individual genes in intracytoplasmic sperm injection rabbit embryos
Author(s) -
Zhang YanLing,
Chen Tao,
Jiang Yan,
Zhong ZhiSheng,
Liu ShuZhen,
Hou Yi,
Schatten Heide,
Chen DaYuan,
Sun QingYuan
Publication year - 2005
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20339
Subject(s) - biology , intracytoplasmic sperm injection , demethylation , dna methylation , methylation , bisulfite , embryo , epigenetics , andrology , bisulfite sequencing , dna demethylation , gene , genetics , microbiology and biotechnology , gene expression , in vitro fertilisation , medicine
Intracytoplasmic sperm injection (ICSI), as an assisted reproduction technique, has been widely used in animal and human. However, its possible effect on epigenetic changes has not been well studied. To investigate whether ICSI can induce aberrant DNA methylation changes in rabbit preimplantation embryos, we examined the methylation status of the SP‐A promoter region and the satellite sequence Rsat IIE by bisulfite‐sequencing technology. The SP‐A promoter region was extensively demethylated before the first round of DNA replication commences, and the unmethylated status was maintained until morula when dynamic remethylation occurred. A similar but more moderate demethylation process was observed in satellite sequence Rsat IIE . These results are in contrast with the previous reports of no active demethylation in normal rabbit embryos, suggesting that the active demethylation we observed may be induced by ICSI. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.