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Production of inbred and hybrid transgenic mice carrying large (> 200 kb) foreign DNA fragments by intracytoplasmic sperm injection
Author(s) -
Osada Tomoharu,
Toyoda Atsushi,
Moisyadi Stefan,
Akutsu Hidenori,
Hattori Masahira,
Sakaki Yoshiyuki,
Yanagimachi Ryuzo
Publication year - 2005
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20319
Subject(s) - biology , transgenesis , bacterial artificial chromosome , transgene , genetics , chromosome , gene , inbred strain , dna , clone (java method) , genome , microbiology and biotechnology , reproductive technology , embryogenesis
We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.