Production of inbred and hybrid transgenic mice carrying large (> 200 kb) foreign DNA fragments by intracytoplasmic sperm injection | Zendy

Osada Tomoharu | Zendy; Toyoda Atsushi | Zendy; Moisyadi Stefan | Zendy; Akutsu Hidenori | Zendy; Hattori Masahira | Zendy; Sakaki Yoshiyuki | Zendy; Yanagimachi Ryuzo | Zendy

Premium

Production of inbred and hybrid transgenic mice carrying large (> 200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Author(s) -

Osada Tomoharu,

Toyoda Atsushi,

Moisyadi Stefan,

Akutsu Hidenori,

Hattori Masahira,

Sakaki Yoshiyuki,

Yanagimachi Ryuzo

Publication year - 2005

Publication title -

molecular reproduction and development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.745

H-Index - 105

eISSN - 1098-2795

pISSN - 1040-452X

DOI - 10.1002/mrd.20319

Subject(s) - biology , transgenesis , bacterial artificial chromosome , transgene , genetics , chromosome , gene , inbred strain , dna , clone (java method) , genome , microbiology and biotechnology , reproductive technology , embryogenesis

We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research