Premium
Full‐term development of mouse eggs transplanted with male pronuclei derived from round spermatids: The effect of synchronization between male and female pronucleus on embryonic development
Author(s) -
Jiang ManXi,
Lei ZiLi,
Ouyang YingChun,
Zhu ZiYu,
Zheng YueLiang,
Sun QingYuan,
Chen DaYuan
Publication year - 2005
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20299
Subject(s) - pronucleus , male pronucleus , biology , spermatid , andrology , zygote , embryogenesis , embryo , microbiology and biotechnology , sperm , genetics , medicine
Pronucleus transplanted mice have been produced, but their donor male pronuclei were derived from mature sperm and were completely synchronous with female pronuclei because both male and female pronuclei came from the same fertilized oocyte. The present study firstly produced male pronuclei by introducing round spermatids into enucleated mouse oocytes, then transferred the male pronuclei into mouse oocytes at three activation stages and finally compared the effect of three kinds of oocytes on the development of reconstructed embryos. Our results indicate that, in enucleated oocytes, mouse round spermatid nuclei can transform to male pronuclei in a higher proportion, and the synchronization between male and female pronucleus does not significantly influence the early cleavage but the later and full‐term development of reconstructed embryos. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.