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Unequal segregation of parental chromosomes in embryonic stem cell hybrids
Author(s) -
Matveeva Natalia M.,
Pristyazhnyuk Inna E.,
Temirova Symbat A.,
Menzorov Alexey G.,
Vasilkova Anna,
Shilov Alexander G.,
Smith Austin,
Serov Oleg L.
Publication year - 2005
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20266
Subject(s) - biology , somatic cell , genetics , homologous chromosome , embryonic stem cell , hybrid , karyotype , cell fusion , chromosome , somatic fusion , microbiology and biotechnology , cell , gene , botany
Chromosome segregation was studied in 14 intra‐ and 20 inter‐specific hybrid clones generated by fusion of Mus musculus embryonic stem (ES) cells with fibroblasts or splenocytes of DD/c mice or Mus caroli. As a control for in vitro evolution of tetraploid karyotype we used a set of hybrid clones obtained by fusion of ES cells (D3) with ES cells (TgTP6.3). Identification of the parental chromosomes in the clones was performed by microsatellite analysis and in situ hybridization with labeled species‐specific probes. Both analyses have revealed three types of clones: (i) stable tetraploid, observed only for ES × ES cell hybrids; (ii) bilateral loss of chromosomes of both ES and somatic partners; (iii) unilateral segregation of chromosomes of the somatic partner. Observed unilateral segregation was extensive in ES–splenocyte cell hybrids, but lower in ES–fibroblast hybrid clones. Developmental state of the somatic partner is presumably responsible for directional chromosome loss. Nonrandom segregation implies that initial differences in the parental homologous chromosomes were not immediately equalized implying at least transient persistence of the differentiated epigenotype. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.