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Lack of RNA–DNA oligonucleotide (chimeraplast) mutagenic activity in mouse embryos
Author(s) -
Tagalakis Aristides D.,
Owen James S.,
Simons J. Paul
Publication year - 2005
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20250
Subject(s) - biology , oligonucleotide , embryo , rna , dna , genetics , microbiology and biotechnology , computational biology , gene
There are numerous reports of the use of RNA–DNA oligonucleotides (chimeraplasts) to correct point mutations in vitro and in vivo, including the human apolipoprotein E gene ( ApoE ). Despite the absence of selection for targeting, high efficiency conversion has been reported. Although mainly used to revert deleterious mutations for gene therapy applications, successful use of this approach would have the potential to greatly facilitate the production of defined mutations in mice and other species. We have attempted to create a point mutation in the mouse ApoE gene by microinjection of chimeraplast into the pronuclei of 1‐cell mouse eggs. Following transfer of microinjected eggs we analysed 139 E12.5 embryos, but obtained no evidence for successful conversion. Mol. Reprod. Dev. 71: 140–144, 2005. © 2005 Wiley‐Liss, Inc.