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Varied patterns of DNA methylation change between different satellite regions in bovine preimplantation development
Author(s) -
Kang YongKook,
Lee HyoJong,
Shim JungJae,
Yeo Seungeun,
Kim SeokHo,
Koo DeogBon,
Lee KyungKwang,
Beyhan Zeki,
First Neal L.,
Han YongMahn
Publication year - 2005
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20249
Subject(s) - biology , reprogramming , methylation , dna methylation , blastocyst , demethylation , satellite , satellite dna , dna demethylation , genetics , microbiology and biotechnology , embryo , dna , embryogenesis , genome , cell , gene , gene expression , engineering , aerospace engineering
Global reduction of DNA methylation, a part of genome reprogramming processes, occurs in a gradual manner until before implantation and is recognized as a conserved process in mammals. Here, we reported that in bovine, satellite regions exhibited varied patterns of methylation changes when one‐cell egg advanced to the blastocyst; a maintenance methylation was observed in satellite I sequences, a decrease in alpha satellites, and an increase in satellite II regions. Cloned embryos exhibited similar changes for DNA methylation in the satellite I and alpha. We also observed that the satellite I and α sequences were methylated more in inner cell mass region of the blastocyst whereas the satellite II showed selective demethylation in this region. Together, these findings point that individual satellite sequences carry their own methylation patterns under the pressure of global demethylation, suggesting that local methylation control system acts on the satellite regions in early bovine embryos. Mol. Reprod. Dev. 71: 29–35, 2005. © 2005 Wiley‐Liss, Inc.