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Vitellogenin mRNA expression in Cherax quadricarinatus during secondary vitellogenic at first maturation females
Author(s) -
SerranoPinto Vania,
Landais Igor,
Ogliastro MarieHelene,
GutiérrezAyala Meliza,
MejíaRuíz Humberto,
VillarrealColmenares Humberto,
GarcíaGasca Alejandra,
VázquezBoucard Celia
Publication year - 2004
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20157
Subject(s) - hepatopancreas , vitellogenin , biology , vitellogenesis , complementary dna , cherax quadricarinatus , microbiology and biotechnology , ovary , northern blot , messenger rna , gene , crayfish , genetics , oocyte , biochemistry , fishery , embryo
PCR products of 1.1 and 0.9 kb were generated using Cherax quadricarinatus genomic DNA in the first case, and hepatopancreas and ovary cDNAs in the second case. These PCR products were cloned and analyzed for nucleotide sequences. The 1.1 kb fragment was used as a probe for Northern hybridization, revealing a transcript of approximately 8 kb in both tissues. Results from both Northern blot and RT‐PCR analyses showed that the mRNA enconding the 3′ end of the vitellogenin cDNA was present simultaneously in both hepatopancreas and ovary tissues in secondary vitellogenic at first maturation females, but was not detected in male hepatopancreas. The deduced amino acid sequences of Vitellogenin (Vg) cDNAs from ovary and hepatopancreas confirmed the existence at least two different Vg genes, and two different sites of synthesis. Mol. Reprod. Dev. 69: 17–21, 2004. © 2004 Wiley‐Liss, Inc.