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Production of transgenic chimeric rabbits and transmission of the transgene through the germline
Author(s) -
Bodó Szilárd,
Gócza Elen,
Révay Tamás,
Hiripi László,
Carstea Bogdan,
Kovács András,
Bodrogi Lilla,
Bösze Zsuzsanna
Publication year - 2004
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20109
Subject(s) - biology , germline , transgene , genetics , transmission (telecommunications) , genetically modified mouse , chimera (genetics) , microbiology and biotechnology , gene , electrical engineering , engineering
Abstract Here we report that improved reproductive technologies combined with an efficient microinjection method and in vitro cultivation medium enabled us to create germ line chimeric rabbits. To follow the fate of the chimeric embryo a blastomere marked with the human blood coagulation factor VIII (hFVIII) transgene was microinjected into a morula stage wild type embryo. The degree of chimerism in different tissues was estimated by real‐time PCR and was found to be in the range of 0.1–42%. Among the four chimeric animals, one was identified as a chromosomal intersex and two were germline chimeras. Mol. Reprod. Dev. 68: 435–440, 2004. © 2004 Wiley‐Liss, Inc.