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Retrovirus‐mediated gene transfer and expression of EGFP in chicken
Author(s) -
Koo Bon Chul,
Kwon Mo Sun,
Choi Bok Ruyl,
Lee Hoon Taek,
Choi Ho Jun,
Kim JinHoi,
Kim NamHyung,
Jeon Iksoo,
Chang Wonkyong,
Kim Teoan
Publication year - 2004
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20102
Subject(s) - biology , green fluorescent protein , blastoderm , rous sarcoma virus , vesicular stomatitis virus , retrovirus , virology , microbiology and biotechnology , embryo , vesicular stomatitis , gene , virus , embryogenesis , genetics
Here, we successfully demonstrate expression of the EGFP (enhanced green fluorescence protein) gene in chickens using replication‐defective MLV (murine leukemia virus)‐based retrovirus vectors encapsidated with VSV‐G (vesicular stomatitis virus G glycoprotein). The recombinant retrovirus was injected beneath the blastoderm of non‐incubated chicken embryos (stage X). After 12 days incubation, all of the eight living embryos assayed were found to express this vector‐encoded EGFP gene, which was under the control of the RSV (Rous Sarcoma Virus) promoter, in diverse organ tissues, including head, beak, neck, wing, hock, tail, toes, heart, amnion, and yolk sac. Surprisingly, despite the presumed cytotoxicity of EGFP, some embryos hatched and survived and these had prominent green fluorescent spots, both in internal organs and externally. Mol. Reprod. Dev. 68: 429–434, 2004. © 2004 Wiley‐Liss, Inc.