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Identification and analysis of stage‐specific expression of lysosome‐associated protein transmembrane 4α gene during development of preimplantation rabbit nuclear transfer embryo
Author(s) -
Yu Weidong,
He Xin,
Liu Guisheng,
Chen Qingxuan
Publication year - 2004
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20094
Subject(s) - biology , embryo , microbiology and biotechnology , in situ hybridization , gene expression , complementary dna , ovary , northern blot , andrology , cdna library , messenger rna , follicle , gene , oocyte , endocrinology , genetics , medicine
Abstract The stage‐specific expression of Lysosome‐associated protein transmembrane 4α (LAPTM4α) in preimplantation rabbit nuclear transfer (NT) embryo was identified with the DDRT‐PCR and reverse Northern Blot. The full length (1,364 bp) cDNA of LAPTM4α was screened out from cDNA library constructed with rabbit ovary and in situ hybridization (ISH) was used to trace the distribution of the LAPTM4α mRNA in intra‐ovary, especially the follicle which proved that the LAPTM4α gene expression is involved in the follicles development, maturation, ovulation, luteinization, and preimplantation development in the rabbit ( Oryctolagus cuniculus domestica ). To our knowledge, this is the first characterization of LAPTM4α gene expression and mRNA distribution in the rabbit ovary and first evidence for this gene involving in follicle development and rabbit preimplantation development. Mol. Reprod. Dev. 68: 415–421, 2004. © 2004 Wiley‐Liss, Inc.