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Histamine enhances cytotrophoblast invasion by inducing intracellular calcium transients through the histamine type‐1 receptor
Author(s) -
Liu Zitao,
Kilburn Brian A.,
Leach Richard E.,
Romero Roberto,
Paria Bibhash C.,
Armant D. Randall
Publication year - 2004
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20082
Subject(s) - histamine , biology , cytotrophoblast , endocrinology , medicine , histamine h2 receptor , blastocyst , trophoblast , phospholipase c , histamine h1 receptor , histamine receptor , receptor , microbiology and biotechnology , signal transduction , placenta , antagonist , biochemistry , pregnancy , embryo , fetus , genetics , embryogenesis
Blastocyst implantation and placentation require molecular and cellular interactions between the uterine endometrium and blastocyst trophectoderm. Previous studies showed that histamine produced in the mouse uterine luminal epithelium interacts with trophoblast histamine type‐2 receptors (H 2 ) to initiate blastocyst implantation. However, it is unknown whether similar histamine activity is operative in humans. Using a human cell line (HTR‐8/SVneo) derived from first‐trimester cytotrophoblasts that expresses both histamine type‐1 receptor (H 1 ) and H 2 , we found that histamine promotes cytotrophoblast invasiveness specifically through activation of H 1 . Stimulation of H 1 in human cytotrophoblasts by histamine induced intracellular Ca 2+ (Ca 2+ i) transients by activating phospholipase C and the inositol trisphosphate pathway. The enhanced invasion induced by histamine was blocked by pretreatment with H 1 antagonist or by chelation of Ca 2+ i. These findings suggest possible differences between rodents and humans in histamine signaling to the trophoblast. Mol. Reprod. Dev. 68: 345–353, 2004. © 2004 Wiley‐Liss, Inc.