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Embryo outcome in Y‐chromosome microdeleted infertile males after ICSI
Author(s) -
Kihaile Paul E.,
Kisanga Ramzy E.,
Aoki Kazuo,
Kumasako Yoko,
Misumi Junichi,
Utsunomiya Takafumi
Publication year - 2004
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20074
Subject(s) - biology , embryo , outcome (game theory) , andrology , chromosome , genetics , gene , medicine , mathematics , mathematical economics
A prospective study involving 118 infertile Japanese couples to assess the embryo outcomes in both azoospermic and oligoasthenoteratoazoospermic (OAT) patients with Y‐chromosome microdeletion. The men were divided into two groups; azoospermia (n = 27), and OAT, sperm concentration <5 × 10 6 /ml (n = 91). They were investigated for Y‐chromosome microdeletions by a polymerase chain reaction (PCR) amplification of the Y‐chromosome‐specific sequence tag site (STS). The embryo outcomes of patients found to have Y‐microdeletion were determined. The frequency of microdeletion was 8.8% (9) and two had microdeletions distal to DAZ. The mean fertilization rate and the cleavage rate in the eight cycles of both azoospermic and oligospermic patients were 59.3 and 87.5%, respectively. The percentages of grade 1 & 2 embryos, ≥6 cells embryos, and blastocyts were 51.7, 65.6, and 45.3%, respectively. Three pregnancies resulted from the eight cycles (37.5%). Conclusion: in Y‐chromosome microdeletion cycles in which sperm cells were available for intracytoplasmic sperm injection (ICSI), embryo outcome was comparable to conventional IVF. Mol. Reprod. Dev. 68: 176–181, 2004. © 2004 Wiley‐Liss, Inc.