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Enriched gonadal migration of donor‐derived gonadal primordial germ cells by immunomagnetic cell sorting in birds
Author(s) -
Kim Jin Nam,
Kim Mi A,
Park Tae Sub,
Kim Duk Kyung,
Park Hyun Jeong,
Ono Tamao,
Lim Jeong Mook,
Han Jae Yong
Publication year - 2004
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20051
Subject(s) - quail , biology , population , andrology , embryo , cell sorting , germ cell , cell , germ , microbiology and biotechnology , endocrinology , genetics , gene , medicine , demography , sociology
This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5‐day‐old chick and 5‐day‐old quail embryos, respectively. Collected gPGCs were loaded into a magnetic‐activated cell sorter (MACS) after being conjugated with specific gPGC antibodies and either MACS‐treated or non‐treated cells in each species were subsequently transferred to the recipient embryos. MACS treatment significantly ( P < 0.05) increased the population ratio of gPGCs in gonadal cells retrieved (0.74 to 33.4% in the chicken and 2.68 to 45.1% in the quail). This was due to decreased number of non‐gPGCs in total cell population. MACS treatment further enhanced gonadal migration of gPGCs transferred in both species (10% vs. 80–85% in the chicken and 10–15% vs. 70–80% in the quail). Increase in the number of microinjected cells up to 600 cells/embryo did not eliminate such promoting effect. In conclusion, MACS treatment greatly increased the population ratio of avian gPGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. Mol. Reprod. Dev. 68: 81–87, 2004. © 2004 Wiley‐Liss, Inc.