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Expression of prostatic secretory protein (PSP)‐like protein in porcine corpus luteum: Isolation and characterization of a new gene encoding PSP 94 ‐like protein
Author(s) -
Tanaka Toshiaki,
Itahana Koji,
Andoh Naoki,
Takeya Tatsuo,
Sato Eimei
Publication year - 1995
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080420204
Subject(s) - biology , corpus luteum , complementary dna , gene , in situ hybridization , cdna library , gene expression , messenger rna , microbiology and biotechnology , ovary , secretory protein , endocrinology , genetics
cDNA clones encoding stage‐and/or space‐specific genes in porcine ovarian follicles have been investigated by differential screening of cDNA libraries of granulosa cells and corpus luteum. A clone corresponding to mRNA preferentially expressed in the functional stage of corpus luteum was found to encode a protein similar to the human prostatic secretory protein, PSP 94 , which had been originally identified in male genital organs. The expression was detected widely in the functional corpora lutea by in situ hybridization histochemistry, but the signal was most prominent in the luteal cells surrounding blood vessels. In contrast, despite the structural similarity in their products, the expression of this newly identified gene was not detected in procine prostate, suggesting that these structurally related proteins play different roles in mammalian genital organs. © 1995 wiley‐Liss, Inc.