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Homeobox gene expression in human oocytes and preembryos
Author(s) -
Verlinsky Yury,
Morozov Gennedy,
Gindilis Viktor,
Strom Charles M.,
Freidin Michael,
Rechitsky Svetlana,
Verlinsky Oleg,
Ivakhnenko Victor,
Zdanovsky Valery,
Kuliev Anver
Publication year - 1995
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080410202
Subject(s) - biology , homeobox , homeobox a1 , embryo , primer (cosmetics) , gene , intron , genetics , reverse transcriptase , microbiology and biotechnology , messenger rna , hnf1b , gene expression , dlx5 , polymerase chain reaction , chemistry , organic chemistry
Homeobox gene expression in human preimplantation development has not been established. We used reverse transcriptase‐polymerase chain reaction (RT‐PCR) with intron spanning primer sets to investigate the presence of mRNA of homeobox genes in human oocytes and preembryos. RT‐PCR products obtained from normal and unfertilized oocytes, from cleaving normal and triploid embryos, and from morulae and blastocysts were cloned, sequenced, and analyzed for the presence of homeobox sequences. The presence of mRNA of homeoboxes HoxA4 and HoxA7 was demonstrated; HoxA4 was present in normal and unfertilized oocytes and also in a 4‐cell embryo. HoxA7 was present in normal oocytes and cleaving triploid embryos. © 1995 Wiley‐Liss, Inc.