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Tissue‐Specific variation in the length of the 5′ untranslated region of the βa‐inhibin mRNA in sheep
Author(s) -
Fleming J. S.,
Galloway S. M.,
Crawford R. J.,
Tisdall D. J.,
Greenwood P. J.
Publication year - 1995
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080400102
Subject(s) - biology , untranslated region , endocrinology , medicine , messenger rna , exon , microbiology and biotechnology , gene , genetics
The 5′ untranslated region (UTR) of βA inhibin mRNA was compared in a variety of sheep tissues, using primer extension. Considerable variation in the length and number of 5′ extended products were noted between tissues. Specific bands were noted in ovarian follicular RNA, which were also present in samples from corpora lutea, stroma, and placental cotyledon RNA. Other extended products were observed in RNA from corpora lutea, stroma, cotyledon, pituitary, bone marrow, frontal cortex, medial basal hypothalamus, adrenal, liver, and kidney, which were not present or weakly represented in follicular RNA, Additional tissue‐specific bands were noted in testis and bone marrow RNA. No specific differences in the lengths of the 5′ UTR of the βA inhibin mRNA were observed in sheep homozygous for the Booroola fecundity gene Fec B , in any tissue studied. The coding region of ovine βA inhibin cDNA was sequenced and a genetic polymorphism confirmed within or close to the ovine βA inhibin gene. We conclude that the βA inhibin gene is expressed widely in the sheep. Furthermore there is variation in the length of the 5′ UTR of βA inhibin mRNA between male and female gonads and other tissues, implying that expression of this gene is differentially controlled. However, the Fec B mutation does not affect mRNA splicing events or the initiation site used in ovarian transcription. The mechanism by which the Fec B mutation influences the amounts of βA inhibin mRNA, follicle‐stimulating hormone (FSH) secretion and ovulation rate has still to be elucidated. © 1995 Wiley‐Liss, Inc.