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Comparison of the ability of progesterone and heat solubilized porcine zona pellucida to initiate the porcine sperm acrosome reaction in vitro
Author(s) -
S. Melendrez Carman,
Meizel Stanley,
Berger Trish
Publication year - 1994
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080390412
Subject(s) - zona pellucida , sperm , acrosome reaction , andrology , biology , fluorescein , capacitation , oocyte , fluorescein isothiocyanate , zona , in vitro , immunology , biochemistry , microbiology and biotechnology , fluorescence , botany , embryo , medicine , virus , physics , quantum mechanics , viral disease
It has been previously shown that progesterone can initiate the acrosome reaction (AR) of capacitated human and hamster sperm in vivo. We report here that progesterone can initiate a morphologically normal AR in porcine sperm that have undergone capacitation in a Hepes‐buffered medium in vitro. In addition, we have compared the abilities of progesterone and heat‐solubilized porcine zona pellucida (zona) to initiate the porcine sperm AR. Capacitated porcine sperm were treated with 1 m̈g/ml progesterone, 150 m̈g/ml porcine zona, or solvent control for 10 min. After treatment, sperm were incubated with the supravital dye Hoechst 33258, fixed and the acrosomal status determined in the previously viable sperm by fluorescence microscopy with fluorescein isothiocyanate‐labeled Pisum sativum agglutini (FITC‐PSA). There was no significant difference between the percentage of AR initiated by zona compared to that initiated by progesterone. In order to determine whether there was a synergistic interaction between the two AR initiators, both were added simultaneously to capacitated porcine sperm at optimal (1 m̈g/ml progesterone, 150 m̈g/ml zona) and suboptimal (75 ng/ml progesterone and 75 m̈g/ml zona) concentrations. Simultaneous addition of the two AR‐initiators at the two concentrations stimulated an additive AR‐initating response, rather than a synergistic one. Several possible explanations for the additive results are discussed. © 1994 Wiley‐Liss, Inc.