Implication that potassium flux and increase in intracellular calcium are necessary for the initiation of sperm motility in salmonid fishes | Zendy

Tanimoto Satomi | Zendy; Nakazawa Tohru | Zendy; Kudo Yoshihisa | Zendy; Morisawa Masaaki | Zendy

Premium

Implication that potassium flux and increase in intracellular calcium are necessary for the initiation of sperm motility in salmonid fishes

Author(s) -

Tanimoto Satomi,

Nakazawa Tohru,

Kudo Yoshihisa,

Morisawa Masaaki

Publication year - 1994

Publication title -

molecular reproduction and development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.745

H-Index - 105

eISSN - 1098-2795

pISSN - 1040-452X

DOI - 10.1002/mrd.1080390409

Subject(s) - sperm , motility , intracellular , biology , sperm motility , calcium in biology , calcium , efflux , intracellular ph , potassium , andrology , microbiology and biotechnology , biochemistry , medicine , chemistry , genetics , organic chemistry

Flux of K + and changes in intracellular Ca 2+ in the sperm of salmonid fishes were measured with spectrophotometry, ion electrode, microscopic fluorometry, and radioisotope accumulation. Release of K + occurred at the initiation of sperm motility which is induced by decrease in external K + and the K + efflux and sperm motility were inhibited by K + channel blockers. Intracellular Ca 2+ increased within a short period in K + ‐ free condition, and the accumulation of 45 Ca in sperm cells was higher in motile sperm than that in immotile sperm. The efflux of K + and the increase in intracellular Ca 2+ were suppressed when external K + concentration increased, i.e., sperm remained immotile. These results suggest that efflux of K + through K + channel and subseqent increase in intracellular Ca 2+ are prerequisite for the initiation of sperm motility. © 1994 Wiley‐Liss, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research