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T‐cell functional regions of the human IL‐3 proximal promoter
Author(s) -
R. Ryan G.,
A. Vadas M.,
F. Shan M.
Publication year - 1994
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080390213
Subject(s) - biology , promoter , repressor , microbiology and biotechnology , gene , transcription (linguistics) , transcription factor , jurkat cells , reporter gene , activator (genetics) , heterologous , response element , gene expression , regulation of gene expression , transcriptional regulation , general transcription factor , genetics , t cell , linguistics , philosophy , immune system
The human interleukin‐3 (IL‐3) gene is expressed almost exclusively in activated T cells. Its expression is regulated at both the transcriptional and post‐transcriptional level. We have previously shown that treatment of Jurkat T cells with phytohemaglutinin (PHA) and the phorbol ester, PMA, activated transcription initiation from the lL‐3 gene. To define the regions of the gene required for transcription activation, we generated a series of reporter constructs containing different regions of the IL‐3 gene 5′ and 3′flanking sequences. Both positive and negative regulatory elements were identified in the proximal 5′ flanking region of the lL‐3 gene. The promoter region between –173 and –60 contained the strongest activating elements. The transcription factor AP‐1 could bind to this positive activator region of the promoter. We also examined the function of the lL‐3 CK‐1/CK‐2 elements that are present in many cytokine genes and found that they acted as a repressor of basal level expression when cloned upstream of a heterologous promoter but were also inducible by PMA/PHA. © 1994 Wiley‐Liss, Inc.