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Cloning and tissue expression of bovine follistatin cDNA
Author(s) -
Houde A.,
Lussier J. G.,
Ethier J.F.,
Gag C.,
Silversides D. W.
Publication year - 1994
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080370405
Subject(s) - follistatin , biology , complementary dna , open reading frame , microbiology and biotechnology , cloning (programming) , start codon , messenger rna , northern blot , nucleic acid , stop codon , rna , rapid amplification of cdna ends , reverse transcriptase , molecular cloning , amino acid , peptide sequence , gene , genetics , computer science , programming language
Bovine follistatin cDNA sequences were derived using a cloning strategy based entirely on reverse transcription and polymerase chain reaction amplification of RNA derived from bovine ovarian and testicular tissues. Complete bovine follistatin cDNA coding sequences are presented including 1,029 bases of open reading frame, the 5′ translational start codon, and the 3′ translational stop codon. Homologies of bovine follistatin cDNA with pig, human, rat, and partial sheep sequences are 94.3%, 92.4%, 89.9%, and 98.4% at the nucleic acid level and 98.3%, 97.1%, 95.6%, and 100% at the deduced amino acid level, respectively. Northern blot analysis on a survey of bovine reproductive tissues showed strongest expression in ovaries collected from superovulated cows and major RNA species at 2.8 Kb and 1.75 Kb. © 1994 Wiley‐Liss, Inc.