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Relationship between sperm nucleus remodelling and cell cycle progression of fragments of mouse parthenogenotes
Author(s) -
Szöllösi Maria S.,
Borsuk Ewa,
Szöllösi Daniel
Publication year - 1994
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080370205
Subject(s) - biology , nucleus , sperm , pronucleus , zygote , cytoplasm , microbiology and biotechnology , chromatin , nucleolus , male pronucleus , cell nucleus , human fertilization , nuclear lamina , embryo , genetics , nuclear protein , embryogenesis , dna , transcription factor , gene
Nucleate and anucleate fragments of parthenogenetically activated mouse oocytes, as well as cybrids obtained by fusion of anucleate fragments (cytoplasts) of maturing and activated matured oocytes were fertilized at different time after activation. Remodelling of the sperm nucleus was studied by electron microscopy at 1.5 and 3 h after fertilization and, in addition, at 14 h in cybrids. Results show that (1) the nuclear envelope of the sperm nucleus can break down when the insemination takes place after the end of M‐phase, but the capacity of the parthenote cytoplasm to remodel the sperm nucleus is restricted in time. (2) Male chromatin can decondense within the old, unbroken nuclear envelope, but in such cases formation of a male pronucleus, one of the two nuclei of zygote possessing inactive nucleoli, is never observed. © 1994 Wiley‐Liss, Inc.