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Sex determination of in vitro developed buffalo ( Bubalus bubalis ) embryos by DNA amplification
Author(s) -
Rao K. B. C. Appa,
Pawshe C. H.,
Totey S. M.
Publication year - 1993
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080360302
Subject(s) - bubalus , biology , embryo , andrology , dna , in vitro , in vitro fertilisation , genetics , ecology , medicine
Abstract This study was conducted to determine the sex of buffalo embryos produced in vitro by amplifying male specific DNA sequences using the polymerase chain reaction (PCR). This method uses three different pairs of bovine Y‐chromosome specific primers and a pair of bovine satellite specific primers. Buffalo in vitro fertilized embryos at the 4‐cell to blastocyst stage were collected at days 3, 4, 6, and 8 postinsemination, and the sex of each embryo was determined using all three different Y‐chromosome specific primers. The bovine satellite sequence specific primers recognize similar sequences in buffalo and are amplified both in males and in females. Similarly, Y‐chromosome specific primers amplify the similar Y‐chromosome specific sequences in male embryos of buffalo. Upon examining genomic DNA from lymphocytes of adult males and females, and embryos, the results demonstrate the feasibility of embryo sexing in buffaloes. Furthermore, sex determination by PCR was found to be a rapid and accurate method. © 1993 Wiley‐Liss, Inc.