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Lysophosphatidylcholine disrupts the acrosome of tammar wallaby ( macropus eugenii ) spermatozoa
Author(s) -
Sistina Yulia,
Lin Minjie,
Rodger John C.
Publication year - 1993
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080350310
Subject(s) - acrosome , lysophosphatidylcholine , biology , incubation , acrosome reaction , andrology , sperm , lipid bilayer fusion , ultrastructure , anatomy , membrane , phosphatidylcholine , biochemistry , phospholipid , botany , medicine
The acrosomal status of wallaby spermatozoa was evaluated by light and electron microscopy after incubation in 1–100 μM lysophosphatidylcholine (LPC) for up to 120 min. Treatment with 1 and 10 μM LPC for 120 min did not lead to acrosomal loss, or detectable alteration to the acrosome, as detected by Bryan's staining and light microscopy. Incubation with 25 μM LPC had little effect on acrosomal loss, however statistically significant changes ( P < 0.05) in the acrosomal matrix (altered) were detected after 10‐min incubation by light microscopy. Around 50% of acrosomes were altered after 20‐min incubation in 50 μM LPC ( P < 0.001), and 40% of spermatozoa had lost their acrosome after 60‐min incubation ( P < 0.001). Treatment with 75 and 100 μM LPC led to rapid acrosomal loss from around 50% of spermatozoa within 10 min ( P < 0.001), and by 60 min acrosomal loss was 70–80%. LPC, like the diacylglycerol DiC 8 (1,2‐di‐octanoyl‐ sn ‐glycerol), is thus an effective agent to induce loss of the relatively stable wallaby sperm acrosome, and it also induces changes within the acrosomal matrix. Ultrastructure of the LPC‐treated spermatozoa revealed that the plasma membrane and the acrosomal membranes were disrupted in a manner similar to that seen after detergent treatment (Triton X‐100). There was no evidence of point fusion between the plasma membrane overlying the acrosome and the outer acrosomal membrane. The plasma membrane was the first structure to disappear from the spermatozoa. The acrosomal membranes and matrix showed increasing disruption with time and LPC concentration. Wallaby spermatozoa incubated with LPC at concentrations that induced significant acrosomal loss also underwent a rapid decline in motility that suggested that acrosomal loss may be due to cell damage, rather than a physiological AR. This study concluded that LPC‐induced acrosomal loss from tammar wallaby spermatozoa is due to its action as a natural detergent and not as a phosphoinositide pathway intermediate. The study further demonstrates the unusual stability of the marsupial acrosomal membranes. © 1993 Wiley‐Liss, Inc.