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Incorporation of [ 3 H]inositol into phosphoinositides and inositol phosphates by rabbit blastocysts
Author(s) -
Fahy Mary M.,
Kane Michael T.
Publication year - 1993
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080340407
Subject(s) - inositol , phosphatidylinositol , second messenger system , biology , inositol phosphate , biochemistry , inositol trisphosphate , signal transduction , receptor
Preimplantation rabbit embryos collected at the early morula stage were cultured to blastocysts in the presence of [ 3 H]inositol. The blastocysts were lysed, and both the aqueous and lipid portions were analysed for incorporated radioactivity. Thin‐layer chromatographic separation of the lipid portion indicated that [ 3 H]inositol was incorporated into phosphatidylinositol, phosphatidylinositol 4‐phosphate, and phosphatidylinositol 4,5‐bisphosphate. HPLC anion‐exchange chromatography indicated that [ 3 H]inositol was incorporated into inositol phosphates, including the two second messengers, inositol 1,4,5‐trisphosphate and inositol 1,3,4,5‐tetrakisphosphate, and also inositol monophosphate and inositol 1,4‐bisphosphate. These results provide evidence that rabbit blastocysts may have an active phosphatidylinositol second messenger system, which may be responsive to intrauterine factors or intraembryonic paracrine factors. © 1993 Wiley‐Liss, Inc.