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Cytoplasmic β‐actin promoter produces germ cell and preimplantation embryonic transgene expression
Author(s) -
Sands Arthur T.,
Hansen Thomas N.,
Demayo Francesco J.,
Stanley Louise A.,
Xin Lei,
Schwartz Robert J.
Publication year - 1993
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080340202
Subject(s) - biology , transgene , embryo , embryonic stem cell , gene , genetically modified mouse , microbiology and biotechnology , gamete , gene expression , germ cell , cytoplasm , genetics , sperm
The cytoplasmic β‐actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken β‐actin‐lacZ fusion gene was expressed in adult testes. In addition, five of the ten lines demonstrated transgene expression in the preimplantation mouse embryo. This is the first example of transgene expression at the stages of both gamete and early embryo. Overall, the site or transgene integration appeared to influence transgene expression in adult tissues. © 1993 Wiley‐Liss, Inc.