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Biosynthesis of prostaglandins by human spermatozoa in vitro and their role in acrosome reaction and fertilization
Author(s) -
Roy Ashim C.,
Ratnam Shan S.
Publication year - 1992
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080330311
Subject(s) - biology , acrosome reaction , human fertilization , in vitro fertilisation , acrosome , biosynthesis , microbiology and biotechnology , in vitro , gamete , andrology , biochemistry , sperm , botany , embryo , genetics , enzyme , medicine
Five homogenates of human sperm cells were separately incubated with [ 14 C]arachidonic acid in the presence of reduced glutathione, L‐tryptophan, and haematin as cofactors. The cyclooxygenase products of archidonic acid metabolism were extracted, separated, and measuréd for their radioactivity. The rate of formation of prostaglandin (PG)D 2 , PGE 2 , PGF 2α , 6‐keto PGF 1α , and thromboxane (TX)B 2 were 18.0 ± 1.11, 10.9 ± 0.68, 5.8 ± 0.21, 3.9 ± 0.13 and 6.6 ± 0.52 pmol/10 6 cells/min, respectively. These results are discussed in relation to the hypothesis that cyclooxygenase metabolites of certain polyunsaturated fatty acids play an important part in the sperm acrosome reaction and fertilization. © 1992 Wiley‐Liss, Inc.