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Vitrification of mouse oocytes using short cryoprotectant exposure: Effects of varying exposure times on survival
Author(s) -
Shaw P. W.,
Bernard A. G.,
Fuller B. J.,
Hunter J. H.,
Shaw R. W.
Publication year - 1992
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080330214
Subject(s) - vitrification , cryoprotectant , biology , cryopreservation , oocyte , andrology , hatching , embryo , zoology , microbiology and biotechnology , medicine
The effects on oocyte viability of varying the duration of exposure to cryoprotectants before rapid cooling to – 196°C were examined, using the vitrification protocol of Nakagata. A very short exposure (15 sec) was found to be optimal, resulting in an overall rate of development from vitrified oocytes to hatching blastocysts of 31.8%. Very high rates of survival (77–89%) of oocytes exposed to the cryoprotectant media, but without the vitrification, together with extreme variability in results between straws in the vitrified groups, suggest that losses in viability during vitrification may result from ice damage during devitrification of the medium. (c) 1992 Wiley‐Liss, Inc.