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Detection of chromosome 17‐ and X‐bearing human spermatozoa using fluorescence in situ hybridization
Author(s) -
Han Tie Lan,
Webb Graham C.,
Flaherty Sean P.,
Correll Anthony,
Matthews Colin D.,
Ford Judith H.
Publication year - 1992
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080330211
Subject(s) - sperm , biology , fluorescence in situ hybridization , hybridization probe , in situ hybridization , microbiology and biotechnology , chromosome , in situ , ploidy , molecular probe , dna , biotinylation , y chromosome , satellite dna , fluorescence , genetics , gene , chemistry , gene expression , optics , physics , organic chemistry
Fluorescence in situ hybridization (FISH) with DNA probes specific to chromosomes 17 and the X has been applied to human ejaculated sperm. After sperm nuclei were decondensed with EDTA and DTT, biotinylated alpha satellite DNA probes TR17 and TRX were separately used on preparations from thirteen healthy donors. After hybridization 96% of sperm were labelled with the TR17 probe and 48% of sperm were labelled with the TRX probe. Frequencies of 0.33% disomic 17 and 0.29% disomic X sperm were found. The frequencies of diploid sperm were assessed as 0.37% using the TR17 probe and 0.20% using the TRX probe which labelled only one half of the sperm; after correcting the result from the X‐probe to 0.40% the two frequencies are very similar. © 1992 Wiley‐Liss, Inc.