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Purification and partial characterization of the 17 kDa sperm coating protein from boar seminal plasma
Author(s) -
Moos JiřRí,
Veselskýa Leopold,
Pěknicov´ Jana,
Drahoréd Josef
Publication year - 1992
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080330208
Subject(s) - capacitation , boar , biology , sperm , zona pellucida , acrosome reaction , epididymis , acrosome , antigen , acrosin , andrology , microbiology and biotechnology , immunology , oocyte , embryo , genetics , medicine
Sperm coating proteins of 16, 17, and 19 kDa have been purified from boar seminal plasma. The 17 kDa protein has been identified as an antigen recognized by monoclonal antibody ACR.3 and is thus identical to low molecular mass zona pellucida binding protein from boar spermatozoa (Moos et al., 1990). The 17 and 19 kDa proteins are glycosylated and tend to form hetero‐complexes. The 17 kDa ACR.3 antigen is sequentially released from the sperm cell surface during capacitation and, after induction of the acrosome reaction, the 16 kDa form was also observed. Immunocytochemical studies on boar reproductive tissues have suggested that the seminal vesicle epithelium may be the source of these proteins. © 1992 Wiley‐Liss, Inc.