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A comparison of two autoradiographic methods for detecting radiolabeled nucleic acids in embryos
Author(s) -
Plante Louise,
Pollard John W.,
King W. Allan
Publication year - 1992
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080330205
Subject(s) - biology , nucleic acid , embryo , computational biology , microbiology and biotechnology , genetics
Two methods for preparing embryos for autoradiographic study of newly synthesized nucleic acids are described and compared. The first method consists of rapidly fixing radiolabeled embryos with acetic acid:methanol, spreading them on glass slides and exposing them for 8 days with a photographic emulsion. The second method consists of fixing, embedding in resin, and sectioning the embryos before their exposure with the emulsion for 3 weeks. Both techniques have many applications in studies of early embryonic activity, but the spread technique is very sensitive, simpler, and faster. © 1992 Wiley‐Liss, Inc.