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Rabbit blastocysts accumulate platelet‐activating factor (PAF) and lyso‐PAF in vitro
Author(s) -
Jones M. A.,
Kudolo G. B.,
Harper M. J. K.
Publication year - 1992
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080320309
Subject(s) - platelet activating factor , biology , platelet activating factor receptor , receptor , in vitro , microbiology and biotechnology , phospholipid , medicine , endocrinology , paracrine signalling , endometrium , phosphorylcholine , andrology , biochemistry , membrane , antagonist
Abstract Platelet‐activating factor (1‐ O ‐alkyl‐2‐acetyl‐ sn ‐glycero‐3‐phosphorylcholine; PAF) is a very potent phospholipid, which has been demonstrated to stimulate smooth muscle and change vascular permeability. PAF has been detected in the rabbit preimplantation uterine endometrium and has been demonstrated to bind specifically to rabbit uterine membranes. To evaluate the possible role of PAF in maternal‐embryonic chemical communication, we report here that rabbit blastocysts can accumulate [ 3 H]PAF from their environment. Blastocysts were able to accumulate [ 3 H]PAF as time‐, buffer‐, age‐, and concentration‐dependent functions. The accumulation was inhibited by some PAF receptor antagonists, such as U66985, as well as by unlabeled PAF and lyso‐PAF, indicating that the accumulation process may be receptor mediated. The data support the current model of PAF as a paracrine factor in preimplantation stages of reproduction.

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