Premium
Localization of ras proto‐oncogene expression during development in Xenopus Laevis
Author(s) -
Andéol Yannick,
Méchali Marcel,
Hourdry Jacques
Publication year - 1992
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080320302
Subject(s) - xenopus , biology , in situ hybridization , embryo , microbiology and biotechnology , rna , cytoplasm , embryogenesis , oogenesis , oocyte , gene expression , yolk , gene , genetics , ecology
The expression of the ras protooncogene was investigated in Xenopus laevis , throughout development, by in situ hybridization using a 35 S‐labelled antisense RNA probe. During oogenesis, the ras RNA was strongly expressed in the cytoplasm of previtellogenic oocytes and further diluted between yolk platelets; no specific localization of transcripts was observed. The signal density was particularly weak over embryo sections until the tailbud stage. On the other hand, a high level of ras RNA expression was detected on sections through the young tadpoles. An intense labelling was observed in several areas, including the branchial apparatus, gut, somites, nervous system, and lens. It is noteworthy that the heterogeneity of labelling increases as tadpoles grow older. Together, these results are discussed in relation to cellular events appearing throughout the early development.